Coding

Part:BBa_K2172011

Designed by: Bowen Xiao   Group: iGEM16_CIEI-BJ   (2016-10-14)


Promoter-RBS-GST-Thrombin protease-SmCPS1-TEV-GFP

This part is a gene circuit that can be used to produce the enzyme SmCPS1. SmCPS1 is a key enzyme on the biosynthesis pathway of tanshinone (a compound used to treat cardiovascular diseases). The tac promoter, a strong hybrid promoter, is used to control and increase the expression levels of the target gene (SmCPS1), and is used in the expression of recombinant proteins, produced from the combination of promoters from the trp and lac operons. The GST label is used to purify the recombinant protein. The GFP is used to measure the expression level of the part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2654
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1415
    Illegal BamHI site found at 747
    Illegal BamHI site found at 1013
    Illegal XhoI site found at 1149
    Illegal XhoI site found at 1173
    Illegal XhoI site found at 2466
    Illegal XhoI site found at 2676
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 818
    Illegal NgoMIV site found at 2235
    Illegal NgoMIV site found at 2799
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159


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